A spline is a continuous floating tongue in a tongue and double groove joint.
Biscuits are interrupted splines in double interrupted grooves.
Both are versions of a mortise and tenon joint, the mortise and tenon being typically much longer and deeper.
The strength of all 3 is that the wooden spline/biscuit/tenon provides a strong mechanical barrier to vertical shear forces as those forces have to shear the wood and the glue, In a butt joint the glue would have 2 planes of shear failure, both horizontally and vertically. Biscuit/spline/mortise joints have mostly only one shear plane which is parallel to the face of the biscuit/spline/tenon or horizontal shear. Obviously, the longer this plane is, the greater the resistance to shear failure. That's the engineering explanation.